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rabbit polyclonal antibodies against zo 1  (Proteintech)


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    Structured Review

    Proteintech rabbit polyclonal antibodies against zo 1
    WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 <t>(ZO-1)</t> expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.
    Rabbit Polyclonal Antibodies Against Zo 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 2520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+zo+1/ZO-1+Antibody/pmc12828522-180-4-11
    Average 97 stars, based on 2520 article reviews
    rabbit polyclonal antibodies against zo 1 - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Engineered Bacillus subtilis WB600/ZD reduces post-weaning diarrhea in piglets by modulating gut microbiota and aryl hydrocarbon receptor (AHR) signaling"

    Article Title: Engineered Bacillus subtilis WB600/ZD reduces post-weaning diarrhea in piglets by modulating gut microbiota and aryl hydrocarbon receptor (AHR) signaling

    Journal: Animal Nutrition

    doi: 10.1016/j.aninu.2025.09.008

    WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 (ZO-1) expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.
    Figure Legend Snippet: WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 (ZO-1) expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.

    Techniques Used: Activation Assay, Infection, Immunohistochemical staining, Expressing, Immunofluorescence, Western Blot, Quantitative RT-PCR, Derivative Assay, Transplantation Assay

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    Incubation:

    Article Title: TNFAIP8 promotes the migration of clear cell renal cell carcinoma by regulating the EMT
    Article Snippet: Equal amount of protein (10-40 μg) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Billerica, MA, USA). .. The membranes were washed and incubated with the following specific primary antibodies at 4°C overnight: rabbit monoclonal antibody against human TNFAIP8 (1:1000; Abcam, ab195810, Suite Cambridge, USA), rabbit polyclonal antibody against E-cadherin (1:5000, Proteintech, 20874-1-AP, Wuhan, Hubei), rabbit polyclonal antibody against N-cadherin (1:2000, Proteintech, 22018-1-AP, Wuhan, Hubei), rabbit polyclonal antibody against ZO-1 (1:500, Proteintech, 21773-1-AP, Wuhan, Hubei), rabbit polyclonal antibody against Vimentin (1:1000, Proteintech, 10366-1-AP, Wuhan, Hubei), and rabbit monoclonal antibody against GAPDH (1:1000; Cell Signaling Technology, #51743, Trask Lane, MA, USA). .. The next day, the membranes were washed and incubated with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG and goat anti-rabbit IgG (1:2000; ZSGB-Bio, Beijing, China) for 1 h at room temperature to visualize the immunoreactive bands.

    Article Title: TNFAIP8 promotes the migration of clear cell renal cell carcinoma by regulating the EMT.
    Article Snippet: Equal amount of protein (10-40 μg) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Billerica, MA, USA). .. The membranes were washed and incubated with the following specific primary antibodies at 4°C overnight: rabbit monoclonal antibody against human TNFAIP8 (1:1000; Abcam, ab195810, Suite Cambridge, USA), rabbit polyclonal antibody against E-cadherin (1:5000, Proteintech, 20874-1-AP, Wuhan, Hubei), rabbit polyclonal antibody against N-cadherin (1:2000, Proteintech, 22018-1-AP, Wuhan, Hubei), rabbit polyclonal antibody against ZO-1 (1:500, Proteintech, 21773-1-AP, Wuhan, Hubei), rabbit polyclonal antibody against Vimentin (1:1000, Proteintech, 10366-1-AP, Wuhan, Hubei), and rabbit monoclonal antibody against GAPDH (1:1000; Cell Signaling Technology, #51743, Trask Lane, MA, USA). ..

    TUNEL Assay:

    Article Title: Effects of Enteromorpha prolifera polysaccharides on growth performance, intestinal barrier function and cecal microbiota in yellow-feathered broilers under heat stress
    Article Snippet: .. The reagents and antibody information used in this section are as follows: TUNEL detection kit (Roche, 11684817910, Switzerland); DAPI (Servicebio, G1012, Wuhan, China); fluorescent labeled primary antibodies contain the rabbit polyclonal antibody against Occludin (GB111401, 1:200 dilution, Servicebio, Wuhan, China), and the rabbit polyclonal antibody against ZO-1 (21773-1-AP, 1:100 dilution, Proteintech, Wuhan, China). .. The secondary antibody is goat polyclonal secondary antibody to rabbit IgG (GB25303, 1:400 dilution, Alexa Fluor® 488, Servicebio, Wuhan, China).

    Article Title: Effects of Enteromorpha prolifera polysaccharides on growth performance, intestinal barrier function and cecal microbiota in yellow-feathered broilers under heat stress.
    Article Snippet: .. The reagents and antibody information used in this section are as follows: TUNEL detection kit (Roche, 11684817910, Switzerland); DAPI (Servicebio, G1012, Wuhan, China); fluorescent labeled primary antibodies contain the rabbit polyclonal antibody against Occludin (GB111401, 1:200 dilution, Servicebio, Wuhan, China), and the rabbit polyclonal antibody against ZO-1 (21773-1-AP, 1:100 dilution, Proteintech, Wuhan, China). .. The secondary antibody is goat polyclonal secondary antibody to rabbit IgG (GB25303, 1:400 dilution, Alexa Fluor® 488, Servicebio, Wuhan, China).

    Labeling:

    Article Title: Effects of Enteromorpha prolifera polysaccharides on growth performance, intestinal barrier function and cecal microbiota in yellow-feathered broilers under heat stress
    Article Snippet: .. The reagents and antibody information used in this section are as follows: TUNEL detection kit (Roche, 11684817910, Switzerland); DAPI (Servicebio, G1012, Wuhan, China); fluorescent labeled primary antibodies contain the rabbit polyclonal antibody against Occludin (GB111401, 1:200 dilution, Servicebio, Wuhan, China), and the rabbit polyclonal antibody against ZO-1 (21773-1-AP, 1:100 dilution, Proteintech, Wuhan, China). .. The secondary antibody is goat polyclonal secondary antibody to rabbit IgG (GB25303, 1:400 dilution, Alexa Fluor® 488, Servicebio, Wuhan, China).

    Article Title: Effects of Enteromorpha prolifera polysaccharides on growth performance, intestinal barrier function and cecal microbiota in yellow-feathered broilers under heat stress.
    Article Snippet: .. The reagents and antibody information used in this section are as follows: TUNEL detection kit (Roche, 11684817910, Switzerland); DAPI (Servicebio, G1012, Wuhan, China); fluorescent labeled primary antibodies contain the rabbit polyclonal antibody against Occludin (GB111401, 1:200 dilution, Servicebio, Wuhan, China), and the rabbit polyclonal antibody against ZO-1 (21773-1-AP, 1:100 dilution, Proteintech, Wuhan, China). .. The secondary antibody is goat polyclonal secondary antibody to rabbit IgG (GB25303, 1:400 dilution, Alexa Fluor® 488, Servicebio, Wuhan, China).



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    WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 <t>(ZO-1)</t> expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.
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    WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 <t>(ZO-1)</t> expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.
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    WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 <t>(ZO-1)</t> expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.
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    Image Search Results


    WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 (ZO-1) expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.

    Journal: Animal Nutrition

    Article Title: Engineered Bacillus subtilis WB600/ZD reduces post-weaning diarrhea in piglets by modulating gut microbiota and aryl hydrocarbon receptor (AHR) signaling

    doi: 10.1016/j.aninu.2025.09.008

    Figure Lengend Snippet: WB600/ZD-FMT and AHR activation alleviate ileal barrier damage caused by Salmonella enterica subsp . enterica serovar Infantis( S. Infantis) infection. (A) Immunohistochemical detection of mucins2 (MUC2) expression in the ileum (the black arrow). (B) Immunofluorescence detection of zonula occludens-1 (ZO-1) expression in the ileum. (C) Claudin-1 and occludin expression in the ileum by Western blot assays. (D-F) RT-qPCR detection of the expression of MUC2 and mucin-related genes trefoil factor 3 ( TFF3 ) and Galactose-3-O-sulfotransferase 2 ( GAL3ST2 ) in the ileum. All data are presented as means ± SD from at least three independent experiments. The P -values shown denote statistical significance for all pairwise comparisons between groups. WB600/ZD is an engineered Bacillus subtilis strain (derived from the protease-deficient parental strain WB600) expressing the antimicrobial defensin peptide from Zophobas atratus , where “ZD” denotes the Zophobas atratus defensin. FMT = fecal microbiota transplantation; SI = S . Infantis infection; CH CH223191. ABX = antibiotic mixture; FICZ = 6-formylindolo[3,2-b]carbazole; PWD = post-weaning diarrhea; AHR = aryl hydrocarbon receptor; CYP1A1 = cytochrome P450 family 1 subfamily A member 1.

    Article Snippet: Staining was performed with rabbit polyclonal antibodies against ZO-1 (21773-1-AP, 1:500; Proteintech Group, Inc., Wuhan, Hubei, China), with tissue sections incubated overnight at 4 °C.

    Techniques: Activation Assay, Infection, Immunohistochemical staining, Expressing, Immunofluorescence, Western Blot, Quantitative RT-PCR, Derivative Assay, Transplantation Assay